The separation and determination of pteroylglutamic acid and related compounds.
نویسندگان
چکیده
Metabolic alteration of certain antagonists of pteroylglutamic acid’ t’o biologically utilizable compounds has been postulated as an explanation of the apparent ability of these analogues t’o promote the growth of a strain of l’etrahymena (1) and an antagonist-resistant strain of Streptococcus faecalis (2). Direct investigation of the possibility that bacterial and leucemic cells, which have become resistant to potent antagonists of folic acid, are able to alter these compounds to metabolically active derivatives required the development of the procedures described herein for the separation of pt,eroylglutamic acid (PGA) from Aminopterin (4-amino-PGA) and A-met,hopterin (4-amino-IO-methyl-PGA). During the use of a bioautographic technique to locate these compounds on paper chromatograms, the occurrence of considerable amounts of growth-promoting, as well as inhibitory, substances as contaminants in the antagonists was observed (3). PGA and pteroic acid were present in samples of Aminopterin and A-methopt’erin in sufficient amounts to account for the apparent “utilization” of these antagonists by a strain of S. faecalis (3, 4). The analysis of a sample of Aminopterin described herein indicated that the cont’ent of PGA was 24 per cent by microbial assay and 22 per cent by fluorometric assay.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 205 1 شماره
صفحات -
تاریخ انتشار 1953